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<h2>Institute of Systematics and Evolution of Animals</h2>
<h4>Polish Academy of Sciences</h4>
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<h3>Head of the Molecular Laboratory</h3>
<p class="fn"><strong>Dr Natalia Sawka-Gądek</strong></p><a href="mailto:[email protected]" class="email">[email protected]</a>
<p class="tel">(48 12) 422 70 06 w. 43</p>
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<li><a href="/en/employees/mkocinski.html">Maciej Kociński</a></li>
<li><a href="/en/employees/nsawka.html">Natalia Sawka-Gądek</a></li>
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<h2>The Laboratory of Molecular Techniques</h2>
<p>The Laboratory of Molecular Techniques provides a general platform within the field of molecular research for scientists and Ph.D. students of all ISEA PAS units. </p>
<p>Our Laboratory offers the possibility to perform analyses such as extraction and evaluation of the quantity and quality of DNA and RNA; DNA amplification by polymerase chain reaction (PCR); DNA sequencing by Sanger method; fragment length analysis by capillary electrophoresis; DNA cloning in biological vectors; preparation of libraries of amplicons and genomic DNA for next-generation sequencing (NGS).</p>
<h3>Equipment</h3>
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<li>ABI 3130xl sequencer</li>
<li>5 thermocyclers: PTC-200 MJ Research, Eppendorf Mastercycler Pro, Eppendorf Mastercycler ProS, SensoQuest Labcycler, Biometra TPersonal</li>
<li>7 centrifuges: Eppendorf 5804 with rotor and deepwell plate centrifuge adaptors, Eppendorf 5430 with plate centrifuge adaptor, Eppendorf 5415D and 5424 with rotor for 24 1.5-2ml tubes and centrifuges with cooling: Eppendorf 5415R and 5424R with 24 tube rotor for 1.5-2ml tubes, Eppendorf 7502R with Swing-Bucket rotor for 15 and 50ml tubes</li>
<li>Spectrophotometer Nanodrop ND-1000</li>
<li>8 electrophoresis devices on agarose gels</li>
<li>Gel archiving system: transilluminator connected to a digital camera and to computer</li>
<li>Shaker with GFL incubator</li>
<li>Scotsman AF100 ice machine</li>
<li>3 laminar chambers</li>
<li>Autoclave</li>
<li>CL-1000 Ultraviolet Crosslinker UVP</li>
<li>Biosan Thermomixer</li>
<li>2 dry heating blocks</li>
<li>3 water baths</li>
<li>Elga Purelab Flex water treatment unit 2</li>
<li>Eppendorf Concentrator plus</li>
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